Cas9 nuclease

  Concentration:

2 mg/ml

  Purity:

Greater than 98.0% as determined by SDS-PAGE.

  Nuclease Activity:

In vivo assay, Cas9 loading with the gRNA cleavage pUC19 plasmid with target DNA insert.

  Amino Acid:

The N terminus contains 6×His-tag and the C-terminus contains TAT peptide for the nuclear localization signal and cell-penetrating peptide(CPP).

  Protein Content Assay:             

Quantification was carried out by Bradford methods

  Non-Specific DNAase:

NA

  Storage Temp:

At -20℃

  Storage Conditions:

In the buffer[20 mM Hepes pH 7.5, 150 mM KCl, 1 mM MgCl2, 5%glycerol, 1 mM TCEP]

  Stability:  

Cas9 although is stable at room temperature, should be stored -20℃. For temporary use, stored 4℃.

  Toxicity:

Cas9 is a polypeptide puritified from culture E. Coli. K-12, nontoxic, noninfectious and nonharzous material.

  Expiry Date:

One year